ING4 Polyclonal antibody

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ING4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10617-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Inhibitor of growth protein 4, p29ING4

• 

  WB analysis using 10617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10617-1-AP (ING4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10617-1-AP

  WB result of ING4 antibody (10617-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING4 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10617-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10617-1-AP (ING4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10617-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10617-1-AP (ING4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10617-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10617-1-AP (ING4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 10617-1-AP

  IP result of anti-ING4 (IP:10617-1-AP, 3ug; Detection:10617-1-AP 1:200) with HEK-293 cells lysate 2700ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 10617-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10617-1-AP (ING4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10617-1-AP targets ING4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0960

  Product name: Recombinant human ING4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-249 aa of BC007781

  Sequence: MAAGMYLEHYLDSIENLPFELQRNFQLMRDLDQRTEDLKAEIDKLATEYMSSARSLSSEEKLALLKQIQEAYGKCKEFGDDKVQLAMQTYEMVDKHIRRLDTDLARFEADLKEKQIESSDYDSSSSKGKKKGRTQKEKKAARARSKGKNSDEEAPKTAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCSIEWFHFACVGLTTKPRGKWFCPRCSQERKKK

• Full Name: inhibitor of growth family, member 4
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007781
• Gene Symbol: ING4
• Gene ID (NCBI): 51147
• RRID: AB_2127940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UNL4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion. This antibody is a rabbit polyclonal antibody raised against full length human ING4 antigen.

Publications

What published studies show

The ING4 antibody (catalog #10617-1-AP) has 14 total citations. Citations appear in Blood, Cancer Research, Advanced Science, Oncogene, Respiratory Research, Molecular Medicine Reports, Brain Pathology, Carcinogenesis, Immunology and Cell Biology, Molecular and Cellular Biology, Pathology-Oncology Research, BMC Research Notes, International Journal of Clinical and Experimental Pathology, and Heliyon. Research fields span hematology, prostate/lung/melanoma/colorectal cancer, pulmonary fibrosis, neuro-oncology, NF-κB-mediated inflammation, and microRNA regulation.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Blood; The Hbo1-Brd1/Brpf2 complex is responsible for global acetylation of H3K14 and required for fetal liver erythropoiesis.; Authors - Mishima Yuta Y
mouse	IHC	Cancer Res; Transient Induction of ING4 by MYC Drives Prostate Epithelial Cell Differentiation and its Disruption Drives Prostate Tumorigenesis.; Authors - Penny L Berger
human	WB,IP,IHC,IF	Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
human	WB	Oncogene; Aberrant CREB1 activation in prostate cancer disrupts normal prostate luminal cell differentiation.; Authors - M J Watson
mouse,human	IHC	Respir Res; Down-regulation of the inhibitor of growth family member 4 (ING4) in different forms of pulmonary fibrosis.; Authors - Tzouvelekis Argyris A
human	IHC	Brain Pathol; Loss of inhibitor of growth (ING-4) is implicated in the pathogenesis and progression of human astrocytomas.; Authors - Klironomos George G