ING4 Monoklonaler Antikörper

ING4 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A12A3

Kat-Nr. : 67754-1-Ig

Synonyme

ING4, Inhibitor of growth protein 4, my036, p29ING4

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Galerie der Validierungsdaten

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  WB analysis using 67754-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of pig brain using 67754-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit brain using 67754-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67754-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67754-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67754-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67754-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 67754-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 67754-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ING4 antibody (67754-1-Ig, Clone: 1A12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Kaninchenhirngewebe, MOLT-4-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67754-1-Ig bindet in WB, IHC, IF, ELISA ING4 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ING4 fusion protein Ag4610
• Vollständiger Name: inhibitor of growth family, member 4
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC007781
• Gene symbol: ING4
• Gene ID (NCBI): 51147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion.