ING3 Polyklonaler Antikörper

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• KD/KO Validated

ING3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10905-1-AP

Synonyme

Eaf4, ING2, ING3, Inhibitor of growth protein 3, p47ING3

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Galerie der Validierungsdaten

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  WB analysis of mouse uterus using 10905-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 10905-1-AP (ING3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10905-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10905-1-AP (ING3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10905-1-AP (ING3 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Uterusgewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Produktinformation

10905-1-AP bindet in WB, IP, IHC, ELISA ING3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ING3 fusion protein Ag1357
• Vollständiger Name: inhibitor of growth family, member 3
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC009777
• Gene symbol: ING3
• Gene ID (NCBI): 54556
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.

Publikationen

Species	Application	Title
human	IHC	Clin Cancer Res; Prognostic significance of nuclear ING3 expression in human cutaneous melanoma.; Authors - Wang Yemin Y
human	WB	Oncogene; The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system.; Authors - Chen G G ; KD Validated
human	WB	Mol Cell Biol; p90 RSK2 mediates antianoikis signals by both transcription-dependent and -independent mechanisms.; Authors - Jin Lingtao L
human	IHC	Front Oncol; Nuclear ING3 Expression Is Correlated With a Good Prognosis of Breast Cancer.; Authors - Xiaoyan Wu
human	WB	Mol Cancer Res; RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival.; Authors - Guoyu Yu
human	WB,IHC	Indian J Med Res; Downregulated inhibitor of growth 3 (ING3) expression during colorectal carcinogenesis.; Authors - Wen-Feng Gou