IMPDH2 Polyklonaler Antikörper

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• KD/KO Validated

IMPDH2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

51

Kat-Nr. : 12948-1-AP

Synonyme

IMPDH II, IMPDH 2, IMPD2, IMPD 2, IMP dehydrogenase II

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Galerie der Validierungsdaten

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  WB analysis of A549 using 12948-1-AP

  WB result of IMPDH2 antibody (12948-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH2 transfected A549 cells.

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  WB analysis using 12948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 12948-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12948-1-AP

  IP result of anti-IMPDH2 (IP:12948-1-AP, 4ug; Detection:12948-1-AP 1:20000) with HeLa cells lysate 1320 ug.

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  IHC staining of human lung cancer using 12948-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12948-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12948-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMPDH2 antibody (12948-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, K-562-Zellen, MCF-7-Zellen, Rattenherzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 30 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Produktinformation

12948-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA IMPDH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IMPDH2 fusion protein Ag3632
• Vollständiger Name: IMP (inosine monophosphate) dehydrogenase 2
• Berechnetes Molekulargewicht: 514 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC012840
• Gene symbol: IMPDH2
• Gene ID (NCBI): 3615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may have cross-reaction with IMPDH1 due to the high homology.

Publikationen

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
mouse	IF	J Clin Invest; Hirschsprung-like disease is exacerbated by reduced de novo GMP synthesis.; Authors - Jonathan I Lake
human	WB	Mol Ther; IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.; Authors - Hailong Ruan ; KD Validated
human	IF	Autoimmun Rev; Anti-rods/rings autoantibodies in a patient with pancreatic injury.; Authors - Shingo Katayama
human	RIP	Cancer Lett; Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer.; Authors - Shiyu Wang
human	WB	Elife; SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome.; Authors - Michela Zaffagni