IL-33 Polyklonaler Antikörper

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• KD/KO Validated

IL-33 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 12372-1-AP

Synonyme

C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

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Galerie der Validierungsdaten

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  WB analysis using 12372-1-AP

  PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL-33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 12372-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL33 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 12372-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12372-1-AP (IL33 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12372-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12372-1-AP (IL33 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, LPS and Brefeldin A treated THP-1 cells, Recombinant protein
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 18 publications below
• IF: See 6 publications below
• ELISA: See 2 publications below

Produktinformation

12372-1-AP bindet in WB, IF, IHC, ELISA IL-33 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-33 fusion protein Ag2957
• Vollständiger Name: interleukin 33
• Berechnetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC047085
• Gene symbol: IL-33
• Gene ID (NCBI): 90865
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Publikationen

Species	Application	Title
mouse	WB	Adv Healthc Mater; Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury; Authors - Liyao Xu
human	IHC,IF	J Invest Dermatol; Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.; Authors - Yi-Giien Tsai
rat	WB	Front Immunol; Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling.; Authors - Hong Xiang
human,mouse	WB,IHC	Am J Pathol; Cross Talk between ST2 and Transforming Growth Factor-β Receptor Signaling Promotes Renal Fibrosis; Authors - Xingxing Zhu
human	WB	EBioMedicine; Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway.; Authors - Shenglong Zhu
human,mouse	IHC	Int Immunopharmacol; Hypoxia induces the production of epithelial-derived cytokines in eosinophilic chronic rhinosinusitis with nasal polyps; Authors - Meiping Zhang