IL-32 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IL-32 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11079-1-AP

Synonyme

IL 32, IL 32alpha, IL 32beta, IL 32delta, IL 32gamma, IL32, IL-32, interleukin 32, Natural killer cells protein 4, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd

---

Galerie der Validierungsdaten

• 

  IHC staining of human lung cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Produktinformation

11079-1-AP bindet in WB, IHC, IF, ELISA IL-32 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-32 fusion protein Ag1484
• Vollständiger Name: interleukin 32
• Berechnetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC009401
• Gene symbol: IL32
• Gene ID (NCBI): 9235
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin 32 (IL32) is a member of the cytokine family. IL32 contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. IL32 is a predominantly intracellular proinflammatory cytokine produced by epithelial cells, monocytes, T-lymphocytes and natural killer cells, and is involved in inflammation and cancer development. IL32 is able to induce the release a variety of pro-inflammatory cytokines such as IL8, tumor necrosis factor-α (TNFα) and IL6. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2.

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; MicroRNA-29b-3p suppresses oral squamous cell carcinoma cell migration and invasion via IL32/AKT signalling pathway.; Authors - Jianya He
human	IHC	J Hepatocell Carcinoma; Identification and Validation of Novel Biomarkers for Hepatocellular Carcinoma, Liver Fibrosis/Cirrhosis and Chronic Hepatitis B via Transcriptome Sequencing Technology.; Authors - Dandan Zhao
human,mouse	IHC,IF	Front Pharmacol; Targeting TIGIT Inhibits Bladder Cancer Metastasis Through Suppressing IL-32.; Authors - Kang Wu
human	WB,IHC	Oncol Lett; Interleukin-32 regulates downstream molecules and promotes the invasion of pancreatic cancer cells.; Authors - Kohji Takagi ; KD Validated
human	WB,IF	Oncol Lett; Establishment of highly invasive pancreatic cancer cell lines and the expression of IL-32.; Authors - Kohji Takagi
human	WB	Biomed Res Int; IL-32 Promotes the Radiosensitivity of Esophageal Squamous Cell Carcinoma Cell through STAT3 Pathway.; Authors - Zhiyu Ma