IL-22RA2 Polyklonaler Antikörper

IL-22RA2 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22861-1-AP

Synonyme

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-453s using 22861-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 22861-1-AP (IL22RA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 22861-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22861-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 22861-1-AP

  1X10^6 HeLa cells were stained with 0.2ug IL22RA2 antibody (22861-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22861-1-AP bindet in WB, IHC, FC,ELISA IL-22RA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-22RA2 fusion protein Ag18854
• Vollständiger Name: interleukin 22 receptor, alpha 2
• Berechnetes Molekulargewicht: 263 aa, 31 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC125167
• Gene symbol: IL22RA2
• Gene ID (NCBI): 116379
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-22 receptor subunit alpha-2 (IL22RA2, also known as CRF2-10) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. It has been described that human IL22RA2 gene encodes three alternatively spliced isoforms called CRF2-10L, CRF2-10, and CRF2-10s, with molecular weights of 31 kDa, 27 kDa, and 15 kDa, respectively.

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