IL-1RA Polyklonaler Antikörper

IL-1RA Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10844-1-AP

Synonyme

IL 1ra, IL1RN, IL-1RN, IL1RA, IL 1RN

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Galerie der Validierungsdaten

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  WB analysis using 10844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 10844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human oesophagus using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10844-1-AP (IL1RA antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of A431 using 10844-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IL-1RA antibody (10844-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 10844-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human IL-1RA (10844-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, MOLT-4-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophagusgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10844-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA IL-1RA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-1RA fusion protein Ag1277
• Vollständiger Name: interleukin 1 receptor antagonist
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC009745
• Gene symbol: IL-1RA
• Gene ID (NCBI): 3557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Clin Transl Oncol; Inhibitory effect and mechanism of mesenchymal stem cells on melanoma cells.; Authors - J Zhang
human	WB	NeuroImmune Pharm Ther; Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages; Authors - Santosh Kumar
human	IHC	Breast Cancer (Dove Med Press); Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer; Authors - Shen Ma
mouse	IF	Front Immunol; The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy; Authors - Yanli Ma