IL-12RB1 Polyklonaler Antikörper

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• KD/KO Validated

IL-12RB1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13287-1-AP

Synonyme

CD212, IL 12 receptor beta component, IL 12 receptor subunit beta 1, IL 12R beta 1, IL 12R BETA1, IL 12R subunit beta 1, IL 12RB1, IL-12 R beta 1, IL12R, IL12RB, IL12RB1, IL-12RB1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 13287-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13287-1-AP (IL12RB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13287-1-AP

  WB result of IL-12RB1 antibody (13287-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12RB1 transfected Jurkat cells.

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  IP experiment of K-562 using 13287-1-AP

  IP result of anti-IL-12RB1 (IP:13287-1-AP, 4ug; Detection:13287-1-AP 1:300) with K-562 cells lysate 1820 ug.

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  IHC staining of human tonsillitis using 13287-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13287-1-AP (IL12RB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

13287-1-AP bindet in WB, IP, IF, IHC, ELISA IL-12RB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-12RB1 fusion protein Ag3983
• Vollständiger Name: interleukin 12 receptor, beta 1
• Berechnetes Molekulargewicht: 662 aa, 73 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC029121
• Gene symbol: IL12RB1
• Gene ID (NCBI): 3594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Publikationen

Species	Application	Title
human, monkey	WB, IP, IF	J Immunol; Inflammatory signals direct expression of human IL12RB1 into multiple distinct isoforms.; Authors - Ford Nicole R NR
mouse	IHC,IF	Front Med (Lausanne); Serum IL-12p40: A novel biomarker for early prediction of minimal change disease relapse following glucocorticoids therapy; Authors - Mengqiu Bai