IL-4 Monoklonaler Antikörper

IL-4 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG1

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

11B11

Kat-Nr. : CL647-65074

Synonyme

Il 4, Il4, IL-4, interleukin 4

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Galerie der Validierungsdaten

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  FC experiment of Mouse Th2-polarized splenocytes using CL647-65074

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) mouse Th2-polarized splenocytes were surface stained with 0.25 ug CoraLite488 Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse IL-4 (CL647-65074, Clone: 11B11).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PMA and ionomycin stimulated mouse Th2-polarized splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65074 bindet in FC (Intra) IL-4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Partially purified native mouse IL-4
• Vollständiger Name: interleukin 4
• GenBank-Zugangsnummer: BC027514
• Gene symbol: Il4
• Gene ID (NCBI): 16189
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C