IKZF1 Polyklonaler Antikörper

IKZF1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 12016-1-AP

Synonyme

DNA binding protein Ikaros, hIk 1, Hs.54452, IK1, IKAROS, IKZF1, LYF1, PRO0758, ZNFN1A1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12016-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12016-1-AP (IKZF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12016-1-AP

  IP result of anti-IKZF1 (IP:12016-1-AP, 4ug; Detection:12016-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human lymphoma using 12016-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12016-1-AP (IKZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12016-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12016-1-AP (IKZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

12016-1-AP bindet in WB, IP, IHC, CoIP, ELISA IKZF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IKZF1 fusion protein Ag2631
• Vollständiger Name: IKAROS family zinc finger 1 (Ikaros)
• Berechnetes Molekulargewicht: 477 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50-70 kDa
• GenBank-Zugangsnummer: BC018349
• Gene symbol: IKZF1
• Gene ID (NCBI): 10320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with 50-70 kDa.

Publikationen

Species	Application	Title
human	CoIP	Circ Res; A Novel Regulatory Mechanism of Smooth Muscle α-actin Expression by NRG-1/circACTA2/miR-548f-5p Axis.; Authors - Yan Sun
human	WB	Cancers (Basel); Mechanistic Basis for In Vivo Therapeutic Efficacy of CK2 Inhibitor CX-4945 in Acute Myeloid Leukemia.; Authors - Morgann Klink
human	WB,CoIP	Eur J Med Chem; Discovery of potential novel CRBN modulators by virtual screening and bioassay.; Authors - Feng Xiong
human	WB	Mol Oncol; Dual inhibition of DNMTs and EZH2 can overcome both intrinsic and acquired resistance of myeloma cells to IMiDs in a cereblon-independent manner.; Authors - Konstantinos Dimopoulos
human	IHC	Leuk Lymphoma; PMID: 29718735; Authors - Konstantinos Dimopoulos
mouse	WB	Eur J Med Chem; Discovery of novel BTK PROTACs with improved metabolic stability via linker rigidification strategy; Authors - Song Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

IKZF1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:12016-1-AP)