IHH Polyklonaler Antikörper

IHH Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 13388-1-AP

Synonyme

BDA1, HHG 2, HHG2, IHH, Indian hedgehog protein

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Galerie der Validierungsdaten

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  WB analysis of human kidney using 13388-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13388-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13388-1-AP (IHH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13388-1-AP (IHH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, HEK-293-Zellen, humanes Herzgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

13388-1-AP bindet in WB, IF, IHC, ELISA IHH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IHH fusion protein Ag4046
• Vollständiger Name: Indian hedgehog homolog (Drosophila)
• Berechnetes Molekulargewicht: 411 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC034757
• Gene symbol: IHH
• Gene ID (NCBI): 3549
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
mouse	IF	Cell Mol Life Sci; Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway.; Authors - Takeshi Oichi
human	IHC	Mol Ther; Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2.; Authors - Hua Wang
mouse	IHC	Osteoarthritis Cartilage; mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis.; Authors - H Zhang
mouse	WB,IHC	Int J Mol Sci; Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism.; Authors - Hongxu Yang
mouse	IHC	Dev Biol; DDB1 promotes the proliferation and hypertrophy of chondrocytes during mouse skeleton development.; Authors - Lianzheng Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hongxue (Verified Customer) (05-13-2022)

WB works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

IHH Antibody Western Blot validation (1:1000 dilution) in Mouse liver (Cat no:13388-1-AP)