IGF2BP3 Rekombinanter Antikörper

IGF2BP3 Rekombinant Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1L11

Kat-Nr. : 81805-1-RR

Synonyme

DKFZp686F1078, hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3

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Galerie der Validierungsdaten

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  WB analysis using 81805-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81805-1-RR (IGF2BP3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 81805-1-RR

  IP result of anti-IGF2BP3(IP:81805-1-RR, 4ug; Detection:81805-1-RR 1:20000) with HeLa cells lysate 1280 ug.

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  IP experiment of HeLa using 81805-1-RR

  IP result of anti-IGF2BP3(IP:81805-1-RR, 4ug; Detection:81805-1-RR 1:4000) with HeLa cells lysate 1280 ug.

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  IHC staining of human pancreas cancer using 81805-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 81805-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 81805-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 81805-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Maus-Plazenta-Gewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81805-1-RR bindet in WB, IP, IHC, ELISA IGF2BP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IGF2BP3 fusion protein Ag6226
• Vollständiger Name: insulin-like growth factor 2 mRNA binding protein 3
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC065269
• Gene symbol: IGF2BP3
• Gene ID (NCBI): 10643
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.