IGF1B-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IGF1B-Specific Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

IHC, IF, Dot blot, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 20215-1-AP

Synonyme

IBP1, IGF I, IGF1, IGF-1, IGF1A, IGF1B, IGF1B-Specific, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C

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Galerie der Validierungsdaten

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  IHC staining of human liver cancer using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20215-1-AP bindet in IHC, IF, Dot blot, ELISA IGF1B-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: insulin-like growth factor 1 (somatomedin C)
• Berechnetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_001111285
• Gene symbol: IGF1
• Gene ID (NCBI): 3479
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IGF1B, also named as IBP1, MGF, IGF-I and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1B.

Publikationen

Species	Application	Title
mouse	Dot blot	Environ Health Perspect; Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.; Authors - Jacob Raber
chicken		Oxid Med Cell Longev; IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.; Authors - Yafan Gong
rat		Cell Death Dis; miR-129 controls axonal regeneration via regulating insulin-like growth factor-1 in peripheral nerve injury.; Authors - Hui Zhu ; KD Validated
human		Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
		Chem Biol Interact; Oenothein B induced human non-small cell lung cancer A549 cell death by ROS-mediated PI3K/Akt/NF-κB signaling pathway.; Authors - Xiaodong Pei
		Mol Cell Biol; Histone Citrullination Represses MicroRNA Expression, Resulting in Increased Oncogene mRNAs in Somatolactotrope Cells.; Authors - Stanley B DeVore