IFT88 Polyklonaler Antikörper

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• KD/KO Validated

IFT88 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-13967

Synonyme

D13S1056E, DAF19, hTg737, IFT88, RP11 172H24.2, TG737, TPR repeat protein 10, TTC10

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Galerie der Validierungsdaten

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  IF Staining of MDCK using CL488-13967

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT88 antibody (CL488-13967) at dilution of 1:200.

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  IF Staining of C2C12 using CL488-13967

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite® Plus 488 IFT88 antibody (CL488-13967) at dilution of 1:200, CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: C2C12-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-13967 bindet in IF IFT88 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFT88 fusion protein Ag4980
• Vollständiger Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 88-95 kDa
• GenBank-Zugangsnummer: BC030776
• Gene symbol: IFT88
• Gene ID (NCBI): 8100
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.