IFT81 Polyklonaler Antikörper

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• KD/KO Validated

IFT81 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11744

Synonyme

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

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Galerie der Validierungsdaten

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  IF Staining of MDCK using CL488-11744

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:50, CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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  IF Staining of hTERT-RPE1 using CL488-11744

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  IF Staining of MDCK using CL488-11744

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11744 bindet in IF IFT81 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFT81 fusion protein Ag2339
• Vollständiger Name: intraflagellar transport 81 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 676 aa, 80 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC029349
• Gene symbol: IFT81
• Gene ID (NCBI): 28981
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.