IFT81 Polyklonaler Antikörper

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• KD/KO Validated

IFT81 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

49

Kat-Nr. : 11744-1-AP

Synonyme

CDV1R, CDV-1, CDV1, CDV 1R, CDV 1

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Galerie der Validierungsdaten

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  WB analysis using 11744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11744-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11744-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11744-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11744-1-AP

  IP result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug.

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  IP experiment of mouse brain using 11744-1-AP

  IP result of anti-IFT81 (IP:11744-1-AP, 4ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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  IHC staining of human prostate cancer using 11744-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11744-1-AP (IFT81 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of hTERT-RPE1 cells using 11744-1-AP

  IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metaphase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim.

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  IF Staining of MDCK using 11744-1-AP

  Immunofluorescent images of MDCK cells stained with IFT81 rabbit pAb (11744-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) for IFT81, and Rhodamine-Goat anti-rabbit IgG for 66200-1-Ig.

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  IF Staining of hTERT-RPE1 cells using 11744-1-AP

  IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.

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  IF Staining of hTERT-RPE1 using 11744-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (11744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  IF Staining of MDCK using 11744-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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  IF Staining of C2C12 using 11744-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, humanes Hirngewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen, hTERT-RPE1-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 32 publications below
• CoIP: See 1 publications below

Produktinformation

11744-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA IFT81 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFT81 fusion protein Ag2339
• Vollständiger Name: intraflagellar transport 81 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 676 aa, 80 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC029349
• Gene symbol: IFT81
• Gene ID (NCBI): 28981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

Publikationen

Species	Application	Title
mouse	IF	Cell; Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.; Authors - Siew Cheng Phua
mouse	IF	Nat Cell Biol; The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment.; Authors - Mu He
human	WB	Nat Cell Biol; Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.; Authors - Malavika Raman
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet
mouse	WB	Nat Commun; Cilia locally synthesize proteins to sustain their ultrastructure and functions.; Authors - Kai Hao
mouse	WB,IF	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan