IFITM2/3 Monoklonaler Antikörper

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• KD/KO Validated

IFITM2/3 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

2E8D12

Kat-Nr. : CL555-66081

Synonyme

1 8U, IFITM3, IP15

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Galerie der Validierungsdaten

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  IF Staining of U2OS using CL555-66081

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®555 IFITM2/3 antibody (CL555-66081, Clone: 2E8D12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-66081

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human IFITM2/3 (CL555-66081, Clone:2E8D12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: U2OS-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66081 bindet in IF, FC (Intra) IFITM2/3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IFITM2/3 fusion protein Ag17863
• Vollständiger Name: interferon induced transmembrane protein 3 (1-8U)
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC070243
• Gene symbol: IFITM3
• Gene ID (NCBI): 10410
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C