IFIT5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IFIT5 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 13378-1-AP

Synonyme

IFIT 5, IFIT5, RI58

---

Galerie der Validierungsdaten

• 

  WB analysis of A375 using 13378-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13378-1-AP (IFIT5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13378-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13378-1-AP (IFIT5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 13378-1-AP

  IP result of anti-IFIT5 (IP:13378-1-AP, 4ug; Detection:13378-1-AP 1:300) with A375 cells lysate 2200ug.

• 

  IHC staining of human skin cancer using 13378-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13378-1-AP (IFIT5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 13378-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13378-1-AP (IFIT5 Antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, PC-3-Zellen
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13378-1-AP bindet in WB, IP, IF, IHC, ELISA IFIT5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFIT5 fusion protein Ag4202
• Vollständiger Name: interferon-induced protein with tetratricopeptide repeats 5
• Berechnetes Molekulargewicht: 482 aa, 58 kDa
• Beobachtetes Molekulargewicht: 56-58 kDa, 48 kDa
• GenBank-Zugangsnummer: BC025786
• Gene symbol: IFIT5
• Gene ID (NCBI): 24138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.

Publikationen

Species	Application	Title
human	WB	J Immunol; IFIT5 Negatively Regulates the Type I IFN Pathway by Disrupting TBK1-IKKε-IRF3 Signalosome and Degrading IRF3 and IKKε.; Authors - Na Zhang
human	WB	Cell Signal; IFIT5 positively regulates NF-κB signaling through synergizing the recruitment of IκB kinase (IKK) to TGF-β-activated kinase 1 (TAK1).; Authors - Caishang Zheng ; KD Validated
Human	WB	Acta Biochim Biophys Sin (Shanghai); IFIT5 potentiates anti-viral response through enhancing innate immune signaling pathways.; Authors - Zhang Bianhong B
human	WB,IHC	Cell Death Dis; The roles and mechanism of IFIT5 in bladder cancer epithelial-mesenchymal transition and progression.; Authors - Jun Huang
human	IHC	Oncoimmunology; Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.; Authors - Chen Zhu
human	WB	mBio; PKR Binds Enterovirus IRESs, Displaces Host Translation Factors, and Impairs Viral Translation to Enable Innate Antiviral Signaling.; Authors - Mikhail I Dobrikov