IFI30 Polyklonaler Antikörper

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• KD/KO Validated

IFI30 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11597-1-AP

Synonyme

IP30, IFI 30, GILT, Gamma-interferon-inducible protein IP-30, Gamma-interferon-inducible lysosomal thiol reductase

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 11597-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11597-1-AP (IFI30 Antibody) at dilution of 1:6000 incubated at 4 degree celsius over night.

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  IP experiment of COLO 320 using 11597-1-AP

  IP result of anti-IFI30 (IP:11597-1-AP, 4ug; Detection:11597-1-AP 1:3000) with COLO 320 cells lysate 2400 ug.

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  IHC staining of human liver cancer using 11597-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11597-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11597-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11597-1-AP (IFI30 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

11597-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA IFI30 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IFI30 fusion protein Ag2178
• Vollständiger Name: interferon, gamma-inducible protein 30
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC021136
• Gene symbol: IFI30
• Gene ID (NCBI): 10437
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IFI30(interferon, gamma-inducible protein 30) is also named as IP30, GILT, IFI-30, MGC32056, IFI30 and belongs to the GILT family. It can facilitate the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. IFI30 is inducibly up-regulated by IFN-g, as are MHC class II molecules, the associated invariant chain, and HLA-DM, which catalyses peptide loading in MIICs and the 35 kDa species is the IFI30 proform, and the 30-kDa species is the mature form(PMID:10639150). It can exsit as a dimer(PMID:17142755).

Publikationen

Species	Application	Title
human	WB,IHC	Int J Med Sci; IFI30 expression predicts patient prognosis in breast cancer and dictates breast cancer cells proliferation via regulating autophagy.; Authors - Yan Fan ; KD Validated
human	WB	Phytomedicine; Nobiletin inhibits de novo FA synthesis to alleviate gastric cancer progression by regulating endoplasmic reticulum stress; Authors - Menglin Chen
human	WB	Aging (Albany NY); Identification and validation of a novel signature based on macrophage marker genes for predicting prognosis and drug response in kidney renal clear cell carcinoma by integrated analysis of single cell and bulk RNA sequencing; Authors - Xiaoxu Chen