IDH2 Polyklonaler Antikörper

IDH2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23254-1-AP

Synonyme

ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase

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Galerie der Validierungsdaten

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  WB analysis using 23254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 23254-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23254-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23254-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23254-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 23254-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 23254-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 23254-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 23254-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23254-1-AP (IDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, DU 145-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslebergewebe, Mausnierengewebe, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

23254-1-AP bindet in WB, IHC, IF, ELISA IDH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IDH2 fusion protein Ag19748
• Vollständiger Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 41-47 kDa
• GenBank-Zugangsnummer: BC009244
• Gene symbol: IDH2
• Gene ID (NCBI): 3418
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved. This antibody is specific to IDH2.

Publikationen

Species	Application	Title
human,mouse	WB,IF	Nat Commun; Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism; Authors - Shubhi Srivastava