ICT1 Polyklonaler Antikörper

ICT1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10403-1-AP

Synonyme

MRPL58, Large ribosomal subunit protein mL62, EC:3.1.1.29, DS-1, DS1

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Galerie der Validierungsdaten

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  WB analysis of A2780 using 10403-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10403-1-AP (ICT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 10403-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10403-1-AP (ICT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SKOV-3 using 10403-1-AP

  IP result of anti-ICT1 (IP:10403-1-AP, 4ug; Detection:10403-1-AP 1:300) with SKOV-3 cells lysate 2000ug.

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  IF Staining of HeLa using 10403-1-AP

  Immunofluorescent analysis of HeLa cells using 10403-1-AP (ICT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

10403-1-AP bindet in WB, IF/ICC, IP, ELISA ICT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ICT1 fusion protein Ag0647
• Vollständiger Name: immature colon carcinoma transcript 1
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 21-24 kDa
• GenBank-Zugangsnummer: BC015335
• Gene symbol: ICT1
• Gene ID (NCBI): 3396
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells. This antibody is a rabbit polyclonal antibody raised against a full-length human ICT1 protein.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Cell Rep; The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly.; Authors - Ya-Ting Tu
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
human	WB	PLoS Genet; RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.; Authors - Aurelio Reyes
human	WB	Mitochondrion; Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.; Authors - Dujeancourt Laurent L
human	WB	Biochem J; The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome.; Authors - Agata Rozanska