Human IgG Polyklonaler Antikörper

Human IgG Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 10284-1-AP

Synonyme

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 10284-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 10284-1-AP (Human IgG antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Human IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Human IgG antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 10284-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG antibody (10284-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 4 publications below

Produktinformation

10284-1-AP bindet in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA Human IgG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Human IgG
• Berechnetes Molekulargewicht: 155 kDa
• Beobachtetes Molekulargewicht: 25 kDa, 50 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This rabbit poly clonal antibody recognizes the human IgG.

Publikationen

Species	Application	Title
	WB	Sci Adv; Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer.; Authors - Long Qin
		Int Immunopharmacol; LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals.; Authors - Zhi-Min Wang
		Front Bioeng Biotechnol; Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.; Authors - Rui-Rui Guo
		Biosensors (Basel); Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber.; Authors - Yijian Huang
	CoIP	Int J Oral Sci; FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.; Authors - Xinyuan Zhao
human	WB	Clin Exp Metastasis; Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer.; Authors - Yahui Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fatima (Verified Customer) (12-11-2018)

• Applications: Flow Cytometry,