HSPBP1 Polyklonaler Antikörper

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• KD/KO Validated

HSPBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10211-1-AP

Synonyme

Hsp70-binding protein 1, Hsp70 interacting protein 2, Hsp70 interacting protein 1, Hsp70 binding protein 2, Hsp70 binding protein 1

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Galerie der Validierungsdaten

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  WB analysis using 10211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10211-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10211-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10211-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10211-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 10211-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10211-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10211-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10211-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10211-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10211-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10211-1-AP (HSPBP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10211-1-AP (HSPBP1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10211-1-AP (HSPBP1 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HeLa using 10211-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPBP1 antibody (10211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, humanes Herzgewebe, humanes Hodengewebe, humanes Skelettmuskelgewebe, Jurkat-Zellen, Maushirngewebe, Maushodengewebe, MCF-7-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

10211-1-AP bindet in WB, IHC, IF/ICC, ELISA HSPBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPBP1 fusion protein Ag0282
• Vollständiger Name: HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC002373
• Gene symbol: HSPBP1
• Gene ID (NCBI): 23640
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hsp70-interacting protein (HSPBP1)is a protein of approximately 40 kilodaltons detected in cell extracts. Its mRNA is present in all tissues analyzed but is most abundant in heart and skeletal muscle. HSPBP1 binds the ATPase domain of Hsp70 and inhibits approximately 90% of the Hsp40-activated Hsp70 ATPase activity by preventing ATP binding to Hsp70.

Publikationen

Species	Application	Title
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
human	WB	Mol Immunol; HSPBP1 facilitates cellular RLR-mediated antiviral response by inhibiting the K48-linked ubiquitination of RIG-I.; Authors - Ya-Xian Yang ; KD Validated