HSPB3 Monoklonaler Antikörper

HSPB3 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A10B5

Kat-Nr. : 67890-1-Ig

Synonyme

Heat shock 17 kDa protein, heat shock 27kDa protein 3, Heat shock protein beta 3, HSP 17, HSPB3, HSPL27

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Galerie der Validierungsdaten

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  WB analysis using 67890-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human heart using 67890-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 67890-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit heart using 67890-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67890-1-Ig (HSPB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 67890-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67890-1-Ig (HSPB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67890-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67890-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPB3 antibody (67890-1-Ig, Clone: 2A10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67890-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human HSPB3 (67890-1-Ig, Clone:2A10B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Kaninchenherzgewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Rattennierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67890-1-Ig bindet in WB, IHC, IF, FC, ELISA HSPB3 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HSPB3 fusion protein Ag26940
• Vollständiger Name: heat shock 27kDa protein 3
• Berechnetes Molekulargewicht: 150 aa, 17 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC126272
• Gene symbol: HSPB3
• Gene ID (NCBI): 8988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.