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HSPA6 Polyklonaler Antikörper
HSPA6 Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (3)
Anwendung
WB, IF, IHC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13616-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HL-60-Zellen, MCF-7-Zellen, U-937-Zellen |
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunhistochemie (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Produktinformation
13616-1-AP bindet in WB, IF, IHC, ELISA HSPA6 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | HSPA6 fusion protein Ag4522 |
| Vollständiger Name | heat shock 70kDa protein 6 (HSP70B') |
| Berechnetes Molekulargewicht | 643 aa, 71 kDa |
| Beobachtetes Molekulargewicht | 68-71 kDa |
| GenBank-Zugangsnummer | BC035665 |
| Gene symbol | HSPA6 |
| Gene ID (NCBI) | 3310 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for HSPA6 antibody 13616-1-AP | Protokoll herunterladen |
| IHC protocol for HSPA6 antibody 13616-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Cell Mol Med ROR2 deficit may induce the tetralogy of Fallot via down-regulating of β-catenin/SOX3/HSPA6 in vitro and in vivo | ||
Am J Cancer Res The stabilization of PD-L1 by the endoplasmic reticulum stress protein GRP78 in triple-negative breast cancer. | ||
Biochim Biophys Acta Mol Cell Res Generation of a pHSPA6 gene-based multifunctional live cell sensor. | ||
Am J Physiol Heart Circ Physiol Molecular mechanisms mediating preconditioning following chronic ischemia differ from those in classical second window. | ||
Evid Based Complement Alternat Med ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling. | ||
Biosci Biotechnol Biochem ARHGEF10L expression regulates cell proliferation and migration in gastric tumorigenesis. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Laureano (Verified Customer) (12-26-2019) | The antibody gave a very clean band without background
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