HSPA13 Polyklonaler Antikörper

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• KD/KO Validated

HSPA13 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12667-2-AP

Synonyme

Heat shock 70 kDa protein 13, HSPA13, STCH

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Galerie der Validierungsdaten

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  WB analysis using 12667-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12667-2-AP (HSPA13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12667-2-AP

  IP result of anti-HSPA13 (IP:12667-2-AP, 4ug; Detection:12667-2-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human endometrial cancer using 12667-2-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12667-2-AP (HSPA13 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

12667-2-AP bindet in WB, IP, IHC, CoIP, ELISA HSPA13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSPA13 fusion protein Ag3349
• Vollständiger Name: heat shock protein 70kDa family, member 13
• Berechnetes Molekulargewicht: 471 aa, 52 kDa
• Beobachtetes Molekulargewicht: 60, 70 kDa
• GenBank-Zugangsnummer: BC036370
• Gene symbol: HSPA13
• Gene ID (NCBI): 6782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSPA13 (Heat shock 70 kDa protein 13), also known as STCH (the stress 70 protein chaperone), is a member of the Hsp70 family of ATPase "stress" or "heat shock" proteins. Human STCH encodes a 60 kDa protein that is highly enriched in the ER. Recently it has been reported that overexpression of the HSPA13 gene reduces prion disease incubation time in mice.

Publikationen

Species	Application	Title
human	WB	Sci Adv; HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.; Authors - Chun Gao ; KO Validated
human	WB	Proc Natl Acad Sci U S A; Overexpression of the Hspa13 (Stch) gene reduces prion disease incubation time in mice.; Authors - Grizenkova Julia J
mouse	WB,CoIP	Front Immunol; Hspa13 Promotes Plasma Cell Production and Antibody Secretion.; Authors - Youdi He
human	WB	Endocrinology; Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.; Authors - Hanshu Wang
human	WB,IHC	Proteome Sci; PRM-based quantitative proteomics analysis of altered HSP abundance in villi and decidua of patients with early missed abortion; Authors - Xiao-Fang Chen
human,mouse	WB,IHC,CoIP	Cell Death Discov; Heat shock protein HSPA13 promotes hepatocellular carcinoma progression by stabilizing TANK; Authors - Xuesong Cen ; KD Validated