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HSP90AB1 Polyklonaler Antikörper

HSP90AB1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11405-1-AP

Synonyme

HSPC3, HSPC2, HSP90B, Heat shock protein HSP 90-beta, Heat shock protein HSP 90 beta



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-3-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe, Maushirngewebe, Maus-Thymusgewebe, MCF-7-Zellen, Rattenherzgewebe, Rattenhirngewebe, Ratten-Thymusgewebe
Erfolgreiche IPNIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Pankreaskarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11405-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA HSP90AB1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HSP90AB1 fusion protein Ag1947
Vollständiger Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Berechnetes Molekulargewicht 723 aa, 83 kDa
Beobachtetes Molekulargewicht 83-90 kDa
GenBank-ZugangsnummerBC014485
Gene symbol HSP90AB1
Gene ID (NCBI) 3326
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Protokolle

Produktspezifische Protokolle
WB protocol for HSP90AB1 antibody 11405-1-APProtokoll herunterladen
IHC protocol for HSP90AB1 antibody 11405-1-APProtokoll herunterladen
IF protocol for HSP90AB1 antibody 11405-1-APProtokoll herunterladen
IP protocol for HSP90AB1 antibody 11405-1-APProtokoll herunterladen
FC protocol for HSP90AB1 antibody 11405-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IP

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
pigWB

Autophagy

Induction of autophagy and suppression of type I IFN secretion by CSFV.

Authors - Baoming Xie
humanWB

Haematologica

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Authors - Stephan Michalik
humanWB

Anal Chem

Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale.

Authors - Xiaolei Zhang
sheepIF

PLoS Pathog

A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion.

Authors - Thomas Labadie
pigWB

Int J Mol Sci

A Comparative Transcriptomic Analysis Reveals That HSP90AB1 Is Involved in the Immune and Inflammatory Responses to Porcine Deltacoronavirus Infection.

Authors - Yujia Zhao
  • KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Maria (Verified Customer) (07-06-2022)

Very good antibody, we achieved good results.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Fibroblasts - human
FH

Kyosuke (Verified Customer) (06-12-2019)

it works good for WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T