HSF4 Polyklonaler Antikörper

HSF4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22883-1-AP

Synonyme

CTM, Heat shock factor protein 4, hHSF4, HSF 4, HSF4, HSTF 4

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 22883-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22883-1-AP (HSF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22883-1-AP (HSF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22883-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22883-1-AP (HSF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22883-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22883-1-AP (HSF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 22883-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22883-1-AP (HSF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 22883-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22883-1-AP (HSF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Mausherzgewebe, Maushirngewebe, Mauslungengewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22883-1-AP bindet in WB, ELISA HSF4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSF4 fusion protein Ag18935
• Vollständiger Name: heat shock transcription factor 4
• Berechnetes Molekulargewicht: 492 aa, 53 kDa
• Beobachtetes Molekulargewicht: 62-66 kDa
• GenBank-Zugangsnummer: BC153061
• Gene symbol: HSF4
• Gene ID (NCBI): 3299
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcriptionlevel. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen, at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. HSF4 exists as two splice variants and is expressed in heart, brain and skeletal muscle as a homotrimer. HSF4a does not contain a DNA-binding domain and inhibits the formation of HSF1 nuclear bodies, thus repressing HSF1 mediated transcription. HSF4b does contain a DNA-binding domain and colocalizes with HSF1 nuclear bodies after heat shock. This antibody is specific to human HSF4.