HSD17B2 Polyklonaler Antikörper

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• KD/KO Validated

HSD17B2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 10978-1-AP

Synonyme

17 beta HSD 2, 17βHSD2, 20 alpha HSD, E2DH, EDH17B2, HSD17, HSD17B2, SDR9C2

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Galerie der Validierungsdaten

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  WB analysis using 10978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10978-1-AP (HSD17B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10978-1-AP

  WB result of HSD17B2 antibody (10978-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B2 transfected HepG2 cells.

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  WB analysis of MCF-7 using 10978-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10978-1-AP (HSD17B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IHC: See 21 publications below

Produktinformation

10978-1-AP bindet in WB, IHC, ELISA HSD17B2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HSD17B2 fusion protein Ag1411
• Vollständiger Name: hydroxysteroid (17-beta) dehydrogenase 2
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC009581
• Gene symbol: HSD17B2
• Gene ID (NCBI): 3294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSD17B2(Estradiol 17-beta-dehydrogenase 2) is also named as 17-beta-HSD 2,20-alpha-HSD,EDH17B2 and belongs to the short-chain dehydrogenases/reductases (SDR) family.Based on the pivotal role of HSD17B2 gene product in the estrogen metabolism pathway, it may be considered as a putative candidate gene that could possibly explain a fraction of the remaining familial breast cancer risk and it is unlikely to play a role as a high penetrance gene in breast cancer predisposition(PMID:18372405).

Publikationen

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
human	IHC	J Clin Endocrinol Metab; MUC1 as a discriminator between endometrium from fertile and infertile patients with PCOS and endometriosis.; Authors - Margarit L L
human	IHC	Br J Cancer; In breast cancer subtypes steroid sulfatase (STS) is associated with less aggressive tumour characteristics.; Authors - Keely M McNamara
human	WB,IHC	Fertil Steril; Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.; Authors - Miao Sun
human	WB,IHC	Endocr Relat Cancer; PMID: 20228125; Authors - Takagi Kiyoshi K
rat	WB	Ecotoxicol Environ Saf; Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression.; Authors - Changjiang Liu