ERAB Polyklonaler Antikörper

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• KD/KO Validated

ERAB Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10648-1-AP

Synonyme

17 beta HSD 10, 17b HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD17B10, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, XH98G2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10648-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10648-1-AP

  WB result of ERAB antibody (10648-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells.

• 

  WB analysis of HeLa using 10648-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10648-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10648-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10648-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10648-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10648-1-AP

  IP result of anti-ERAB (IP:10648-1-AP, 4ug; Detection:10648-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human breast cancer using 10648-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10648-1-AP (ERAB antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A2780-Zellen, HEK-293-Zellen, humanes Hirngewebe, Mauslebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IP: See 1 publications below

Produktinformation

10648-1-AP bindet in WB, IHC, IP, ELISA ERAB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERAB fusion protein Ag1020
• Vollständiger Name: hydroxysteroid (17-beta) dehydrogenase 10
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC008708
• Gene symbol: ERAB
• Gene ID (NCBI): 3028
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.; Authors - Oliver Rackham
human	WB	Hum Mol Genet; Leber's hereditary optic neuropathy (LHON)-associated ND6 14 484 T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.; Authors - Min Liang
rat	WB	J Oleo Sci; Effects of Dietary Fat Restriction on Endurance Training-induced Metabolic Adaptations in Rat Skeletal Muscle.; Authors - Takuya Karasawa
mouse	WB	Circulation; Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation; Authors - Matthew A Walker
rat	WB	Gen Comp Endocrinol; Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats; Authors - Dong Li
human,mouse	WB,IP	Phytother Res; Astragaloside IV protects renal tubular epithelial cells against oxidative stress-induced injury by upregulating CPT1A-mediated HSD17B10 lysine succinylation in diabetic kidney disease; Authors - Meng Wang