HRH1 Polyklonaler Antikörper

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• KD/KO Validated

HRH1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 13413-1-AP

Synonyme

H1 R, H1R, HH1R, hisH1, Histamine H1 receptor, histamine receptor H1, HRH1

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Galerie der Validierungsdaten

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  WB analysis of human colon using 13413-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13413-1-AP (HRH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13413-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13413-1-AP (HRH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 13413-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13413-1-AP (HRH1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human endometrial cancer using 13413-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13413-1-AP (HRH1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human trachea using 13413-1-AP

  Immunohistochemical analysis of paraffin-embedded human trachea using 13413-1-AP (HRH1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 13413-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13413-1-AP (HRH1 antibody) at dilution of 1:50 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Tracheagewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

13413-1-AP bindet in WB, IHC, IP, ELISA, IF HRH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HRH1 fusion protein Ag4067
• Vollständiger Name: histamine receptor H1
• Berechnetes Molekulargewicht: 487 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC060802
• Gene symbol: HRH1
• Gene ID (NCBI): 3269
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Stem Cell Reports; Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids.; Authors - Keiichiro Uehara
human	WB	Aging (Albany NY); Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma.; Authors - Yi-Fang Ding
mouse	WB	Front Cell Neurosci; Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.; Authors - Wen-Jun Su
human	WB,IHC,IF	J Virol; Kaposi's Sarcoma-Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.; Authors - Mohanan Valiya Veettil ; KO Validated
rat	WB	Sci Rep; Gold nanoclusters eliminate obesity induced by antipsychotics.; Authors - Meng He
mouse	IF	Am J Physiol Cell Physiol; Functional role of histamine receptors in the renal cortical collecting duct cells.; Authors - Anastasia V Sudarikova