HPS6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HPS6 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11371-1-AP

Synonyme

Hermansky Pudlak syndrome 6, HPS6, RP11 302K17.1, Ru, Ruby eye protein homolog

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11371-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11371-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11371-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11371-1-AP

  IP result of anti-HPS6 (IP:11371-1-AP, 4ug; Detection:11371-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IHC staining of human ovary tumor using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11371-1-AP (HPS6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11371-1-AP (HPS6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11371-1-AP (HPS6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11371-1-AP (HPS6 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

11371-1-AP bindet in WB, IP, IHC, ELISA HPS6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HPS6 fusion protein Ag1929
• Vollständiger Name: Hermansky-Pudlak syndrome 6
• Berechnetes Molekulargewicht: 775 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC011594
• Gene symbol: HPS6
• Gene ID (NCBI): 79803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hermansky-Pudlak syndrome (HPS) defines a group of at least seven autosomal recessive disorders characterized by albinism and prolonged bleeding due to defects in the lysosome-related organelles, melanosomes and platelet-dense granules, respectively. HSP6, also named as Ru, regulates the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules. It acts as cargo adapter for the dynein-dynactin motor complex to mediate the transport of lysosomes from the cell periphery to the perinuclear region.

Publikationen

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda ; KD Validated
human	WB	EMBO Rep; Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.; Authors - Saurabh Shakya
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook
human	WB	Adv Sci (Weinh); Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase; Authors - Johannes Naß