HPS5 Polyklonaler Antikörper

HPS5 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 13901-1-AP

Synonyme

KIAA1017, Hermansky-Pudlak syndrome 5 protein, Hermansky Pudlak syndrome 5, BLOC-2 complex member HPS5, Alpha-integrin-binding protein 63

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 13901-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13901-1-AP (HPS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13901-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13901-1-AP (HPS5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 13901-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13901-1-AP (HPS5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 13901-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HPS5 antibody 13901-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

13901-1-AP bindet in WB, IHC, IF/ICC, ELISA HPS5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HPS5 fusion protein Ag4867
• Vollständiger Name: Hermansky-Pudlak syndrome 5
• Berechnetes Molekulargewicht: 1129 aa, 127 kDa
• Beobachtetes Molekulargewicht: 135-141 kDa
• GenBank-Zugangsnummer: BC033640
• Gene symbol: HPS5
• Gene ID (NCBI): 11234
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hermansky-Pudlak syndrome 5 protein (HPS-5), also named Ruby-eye protein 2 homolog (Ru2), plays a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. This protein interacts with Hermansky-Pudlak syndrome 6 protein and may interact with the cytoplasmic domain of integrin, alpha-3. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 5.

Publikationen

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda
human	WB	Am J Hum Genet; A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.; Authors - Cullinane Andrew R AR
human	WB	J Invest Dermatol; Clinical, molecular, and cellular features of non-Puerto Rican Hermansky-Pudlak syndrome patients of Hispanic descent.; Authors - Carmona-Rivera Carmelo C
human	WB	Pigment Cell Melanoma Res; A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8.; Authors - Cullinane Andrew R AR
human	WB	PLoS One; Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5.; Authors - Joshi Stephen
human	WB	Front Genet; Oculocutaneous albinism and bleeding diathesis due to a novel deletion in the HPS3 gene; Authors - Dina Marek-Yagel