VILIP3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VILIP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10989-1-AP

Synonyme

HPCAL1, Visinin-like protein 3, VILIP-3, hippocalcin like 1, Calcium-binding protein BDR-1

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Galerie der Validierungsdaten

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  WB analysis using 10989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10989-1-AP (VILIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10989-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10989-1-AP (VILIP 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10989-1-AP

  IP result of anti-VILIP3 (IP:10989-1-AP, 4ug; Detection:10989-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human kidney using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10989-1-AP (VILIP 3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10989-1-AP (VILIP 3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse cerebellum using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10989-1-AP (VILIP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10989-1-AP (VILIP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10989-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VILIP3 antibody (10989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

10989-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA VILIP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VILIP3 fusion protein Ag1449
• Vollständiger Name: hippocalcin-like 1
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC009846
• Gene symbol: VILIP3
• Gene ID (NCBI): 3241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VILIP3, is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system.

Publikationen

Species	Application	Title
human	WB	Autophagy; Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis; Authors - Xin Chen ; KD Validated
mouse	IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
human	WB,IHC	Hepatology; Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization via activating ERK1/2-MAPK pathway.; Authors - Yonglong Zhang ; KD Validated
human	WB,IHC	Cancer Med; Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments.; Authors - Mingjian Ma
human,mouse	WB	Theranostics; Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling; Authors - Tao Chen
human	IHC	Signal Transduct Target Ther; Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression; Authors - Xiang-Peng Tan