HOXC10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HOXC10 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12025-1-AP

Synonyme

HOX3I, Homeobox protein Hox-C10, Homeobox protein Hox-3I

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 12025-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12025-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12025-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 12025-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12025-1-AP (HOXC10 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, A549-Zellen, C2C12-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

12025-1-AP bindet in WB, IHC, IF/ICC, ELISA HOXC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOXC10 fusion protein Ag2655
• Vollständiger Name: homeobox C10
• Berechnetes Molekulargewicht: 342 aa, 38 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC001293
• Gene symbol: HOXC10
• Gene ID (NCBI): 3226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486)

Publikationen

Species	Application	Title
human	IHC	Int Immunopharmacol; HOXC6/8/10/13 predict poor prognosis and associate with immune infiltrations in glioblastoma.; Authors - Mingjun Yu
human	WB,IHC	Chin J Cancer Res; HOXC10 up-regulation promotes gastric cancer cell proliferation and metastasis through MAPK pathway.; Authors - Chen Guo
human	WB,IF	Cell Rep; Identification of cis-HOX-HOXC10 axis as a therapeutic target for colorectal tumor-initiating cells without APC mutations.; Authors - Zhenzhen Chen ; KO Validated