HOXB7 Polyklonaler Antikörper

HOXB7 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12613-1-AP

Synonyme

HOX2C, Homeobox protein Hox-B7, Homeobox protein Hox-2C, Homeobox protein HHO.C1, HHO.C1

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 antibody) at dilution of 1:300.

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  IF Staining of HepG2 using 12613-1-AP

  Immunofluorescent analysis of HepG2 cells using 12613-1-AP (HOXB7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen
• Erfolgreiche IP: SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

12613-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HOXB7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOXB7 fusion protein Ag3245
• Vollständiger Name: homeobox B7
• Berechnetes Molekulargewicht: 217 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24-30 kDa
• GenBank-Zugangsnummer: BC015345
• Gene symbol: HOXB7
• Gene ID (NCBI): 3217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	J Neuroinflammation; Gene expression comparison reveals distinct basal expression of HOX members and differential TNF-induced response between brain- and spinal cord-derived microvascular endothelial cells.; Authors - Yves Molino
human	WB	Int J Oncol; CircRNA hsa_circ_0070934 functions as a competitive endogenous RNA to regulate HOXB7 expression by sponging miR‑1236‑3p in cutaneous squamous cell carcinoma.; Authors - Da-Wei Zhang
human	WB	Exp Cell Res; Long non-coding RNA BBOX1-AS1 exacerbates esophageal squamous cell carcinoma development by regulating HOXB7/β-catenin axis.; Authors - Jinxiu Sheng
human	WB,IHC	Biomed Res Int; Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer; Authors - Jialei Chen