HOXA9 Polyklonaler Antikörper

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• KD/KO Validated

HOXA9 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 18501-1-AP

Synonyme

ABD B, homeobox A9, Homeobox protein Hox 1G, Homeobox protein Hox A9, HOX1, HOX1.7, HOX1G, HOXA9

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Galerie der Validierungsdaten

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  WB analysis of EC109 using 18501-1-AP

  EC109 cells were subjected to SDS PAGE followed by western blot with 18501-1-AP (HOXA9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 18501-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18501-1-AP (HOXA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse appendix using 18501-1-AP

  IP Result of anti-HOXA9 (IP:18501-1-AP, 3ug; Detection:18501-1-AP 1:300) with mouse appendix tissue lysate 4000ug.

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  IHC staining of mouse skin using 18501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18501-1-AP (HOXA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EC109-Zellen, Maus-Kolongewebe
• Erfolgreiche IP: Maus-Appendix-Gewebe
• Erfolgreiche Detektion in IHC: Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

18501-1-AP bindet in WB, IP, IHC, chIP, ELISA HOXA9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOXA9 fusion protein Ag13350
• Vollständiger Name: homeobox A9
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC010023
• Gene symbol: HOXA9
• Gene ID (NCBI): 3205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOXA9, also known as HOX1G, belongs to the Abd-B homeobox family and transiently interacts with PRMT5 in TNF-alpha stimulated endothelial cells. HOXA9 is methylated on Arg-140 by PRMT5and methylation is critical for E-selectin induction. HOXA9 as a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HOXA9 is required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. HOXA9 is 30 kDa, but methylation of HOXA9 is about 35-40 kDa. (PMID: 10082572)

Publikationen

Species	Application	Title
human	WB	Clin Epigenetics; Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line; Authors - Tina Aumer
human	WB	iScience; TIFAB accelerates MLL-AF9-Induced acute myeloid leukemia through upregulation of HOXA9.; Authors - Jinming Zhao
human	WB,chIP	Cell Signal; Aberrant TRPM4 expression in MLL-rearranged acute myeloid leukemia and its blockade induces cell cycle arrest via AKT/GLI1/Cyclin D1 pathway.; Authors - Fujue Wang ; KD Validated
human	WB	Cell Biosci; Hypoxic metabolism in human hematopoietic stem cells.; Authors - Fatih Kocabas
mouse	WB	Cell Rep; YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.; Authors - Jiadong Liu
human	chIP	Sci Rep; Transcription Factor HOXA9 is Linked to the Calcification and Invasion of Papillary Thyroid Carcinoma.; Authors - Yilan Jin