HOMER3 Polyklonaler Antikörper

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• KD/KO Validated

HOMER3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16624-1-AP

Synonyme

HOMER 3, homer homolog 3 (Drosophila), Homer protein homolog 3, HOMER3, VESL3

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 16624-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16624-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16624-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16624-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cerebellum using 16624-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 16624-1-AP (HOMER3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 16624-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 16624-1-AP (HOMER3 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Mauslungengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1200
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

16624-1-AP bindet in WB, IHC, ELISA HOMER3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOMER3 fusion protein Ag9923
• Vollständiger Name: homer homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 361 aa, 40 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC012113
• Gene symbol: HOMER3
• Gene ID (NCBI): 9454
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HOMER3 is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.

Publikationen

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
rat	WB	Neuroscience; Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety.; Authors - Dan Liu
human	IHC	Cell Death Dis; Long non-coding RNA HOMER3-AS1 drives hepatocellular carcinoma progression via modulating the behaviors of both tumor cells and macrophages.; Authors - Jian Pu ; KD Validated