HNRNPUL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HNRNPUL1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10578-1-AP

Synonyme

E1B AP5, E1BAP5, HNRNPUL1, HNRPUL1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10578-1-AP

  WB result of HNRNPUL1 antibody (10578-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPUL1 transfected HEK-293 cells.

• 

  WB analysis of A549 using 10578-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10578-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10578-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10578-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10578-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10578-1-AP

  IP Result of anti-HNRNPUL1 (IP:10578-1-AP, 5ug; Detection:10578-1-AP 1:2000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human ovary tumor using 10578-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:100 (under 25x lens).

• 

  IF Staining of HepG2 using 10578-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10578-1-AP

  Immunofluorescent analysis of HepG2 cells, using HNRNPUL1 antibody 10578-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 10578-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug HNRNPUL1 antibody (10578-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10578-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA HNRNPUL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPUL1 fusion protein Ag0899
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein U-like 1
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 90-115 kDa
• GenBank-Zugangsnummer: BC002564
• Gene symbol: HNRNPUL1
• Gene ID (NCBI): 11100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1-Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4 .

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB,IF,IP	Proc Natl Acad Sci U S A; Influenza virus targets the mRNA export machinery and the nuclear pore complex.; Authors - Satterly Neal N
	WB	PLoS Pathog; Cellular RNA Binding Proteins NS1-BP and hnRNP K Regulate Influenza A Virus RNA Splicing.; Authors - Tsai Pei-Ling PL
human	WB,IF	EMBO Rep; Vesicular stomatitis virus inhibits mitotic progression and triggers cell death.; Authors - Chakraborty Papia P
mouse	WB,IF	J Autoimmun; RNA-binding protein hnRNP UL1 binds κB sites to attenuate NF-κB-mediated inflammation.; Authors - Zhongfei Ma ; KO Validated
human	IHC	Exp Cell Res; HNRNPUL1 inhibits cisplatin sensitivity of esophageal squamous cell carcinoma through regulating the formation of circMAN1A2.; Authors - Juan Li