HNRNPK Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HNRNPK Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

37

Rezensionen (1)

Kat-Nr. : 11426-1-AP

Synonyme

TUNP, Transformation up-regulated nuclear protein, HNRPK, hnRNP K, FLJ41122

---

Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 11426-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11426-1-AP (HNRNPK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11426-1-AP

  IP result of anti-HNRNPK (IP:11426-1-AP, 4ug; Detection:11426-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human breast cancer using 11426-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11426-1-AP (HNRNPK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11426-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11426-1-AP (HNRNPK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11426-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPK antibody (11426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11426-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPK antibody (11426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11426-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HNRNPK (11426-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 29 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below
• RIP: See 7 publications below

Produktinformation

11426-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA HNRNPK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPK fusion protein Ag1976
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein K
• Berechnetes Molekulargewicht: 463 aa, 51 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC014980
• Gene symbol: HNRNPK
• Gene ID (NCBI): 3190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability.

Publikationen

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li ; KD Validated
		Nat Commun; PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.; Authors - Chen-Yu Wang
		Nucleic Acids Res; Capturing RNA-protein interaction via CRUIS.; Authors - Ziheng Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

DEBJIT (Verified Customer) (09-17-2020)

WB: 1:2000IP: 2ug/mg cell lysate

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: Endothelial cells