HNRNPA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HNRNPA1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Rezensionen (2)

Kat-Nr. : 11176-1-AP

Synonyme

HNRPA1, hnRNP core protein A1, hnRNP A1, Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed, Helix-destabilizing protein

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Galerie der Validierungsdaten

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  WB analysis using 11176-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11176-1-AP (HNRNPA1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 11176-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11176-1-AP (HNRNPA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11176-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA1 antibody (11176-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, K-562-Zellen, Maus-Kolongewebe, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 31 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Produktinformation

11176-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HNRNPA1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPA1 fusion protein Ag1656
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein A1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34, 40 kDa
• GenBank-Zugangsnummer: BC009600
• Gene symbol: HNRNPA1
• Gene ID (NCBI): 3178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

Publikationen

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
mouse	WB	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
human	WB	Mol Cell; A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.; Authors - Jin-Zhou Huang ; KD Validated
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li ; KD Validated
pig	WB	Mol Biol Evol; Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.; Authors - Ke Chen
human	WB,IF	J Cell Biol; An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.; Authors - Joseph R Tran

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Good quality. It is easy for you to find the target bind.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

HNRNPA1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11176-1-AP)

FH

Katie (Verified Customer) (04-08-2022)

Works nicely for IF in primary CD4+ T cells. 2hr Primary incubation at 37 and 2hr secondary incubation at 37oc 1:1000.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50