HNRNPA0 Polyklonaler Antikörper

HNRNPA0 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 10848-1-AP

Synonyme

HNRPA0, hnRNP A0

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Galerie der Validierungsdaten

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  WB analysis using 10848-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10848-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 10848-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 10848-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of human brain using 10848-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10848-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10848-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500.

• 

  IF Staining of HeLa using 10848-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10848-1-AP (HNRNPA0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

10848-1-AP bindet in WB, IF/ICC, IP, ELISA HNRNPA0 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPA0 fusion protein Ag1312
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein A0
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC019271
• Gene symbol: HNRNPA0
• Gene ID (NCBI): 10949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	IF	Cell; RNA damage compartmentalization by DHX9 stress granules; Authors - Yilong Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaochen (Verified Customer) (07-31-2023)

hnrnpa0 has many subtypes and thus got two bands, I think it is acceptable. Antibody quality is prety good.

• Applications: Western Blot

HNRNPA0 Antibody Western Blot validation ( dilution) in (Cat no:10848-1-AP)