HNMT Polyklonaler Antikörper

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• KD/KO Validated

HNMT Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11874-1-AP

Synonyme

HNMT S2, HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase

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Galerie der Validierungsdaten

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  WB analysis using 11874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11874-1-AP

  WB result of HNMT antibody (11874-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNMT transfected HepG2 cells.

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  WB analysis of mouse liver using 11874-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11874-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11874-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11874-1-AP (HNMT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11874-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11874-1-AP (HNMT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11874-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11874-1-AP (HNMT antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 11874-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11874-1-AP (HNMT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HepG2-Zellen, humanes Lebergewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11874-1-AP bindet in WB, IHC, IF/ICC, ELISA HNMT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNMT fusion protein Ag2462
• Vollständiger Name: histamine N-methyltransferase
• Berechnetes Molekulargewicht: 292 aa, 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC020677
• Gene symbol: HNMT
• Gene ID (NCBI): 3176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.

Publikationen

Species	Application	Title
human	IHC	Glia; Molecular mechanism of histamine clearance by primary human astrocytes.; Authors - Yoshikawa Takeo T
human	WB	Cell Stem Cell; Glioblastoma stem cell-specific histamine secretion drives pro-angiogenic tumor microenvironment remodeling; Authors - Jiayi Chen