HMGN2 Polyklonaler Antikörper

HMGN2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10953-1-AP

Synonyme

Non-histone chromosomal protein HMG-17, HMG17, High mobility group nucleosome-binding domain-containing protein 2

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 10953-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10953-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10953-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10953-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10953-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10953-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10953-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• ELISA: See 1 publications below

Produktinformation

10953-1-AP bindet in WB, IHC, IF/ICC, ELISA HMGN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGN2 fusion protein Ag1401
• Vollständiger Name: high-mobility group nucleosomal binding domain 2
• Berechnetes Molekulargewicht: 9 aa, 3 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC014644
• Gene symbol: HMGN2
• Gene ID (NCBI): 3151
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.

Publikationen

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance; Authors - Mengting Xu
human	ELISA	Mol Cancer; HMGN2, a new anti-tumor effector molecule of CD8+ T cells.; Authors - Lin Su
pig	WB	J Proteomics; DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase; Authors - Chen Zhou