HMGN1 Polyklonaler Antikörper

HMGN1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11695-1-AP

Synonyme

Non-histone chromosomal protein HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 11695-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11695-1-AP (HMGN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11695-1-AP (HMGN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11695-1-AP (HMGN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11695-1-AP

  Immunofluorescent analysis of HepG2 cells, using HMGN1 antibody 11695-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

11695-1-AP bindet in WB, IHC, IF/ICC, ELISA HMGN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGN1 fusion protein Ag2315
• Vollständiger Name: high-mobility group nucleosome binding domain 1
• Berechnetes Molekulargewicht: 100 aa, 11 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC023984
• Gene symbol: HMGN1
• Gene ID (NCBI): 3150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMGN1 (high-mobility group nucleosome-binding protein 1) is a member of the HMG superfamily of nonhistone chromatin-binding proteins, which which regulate chromatin structure and gene transcription. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. It may participate in the process which maintains transcribable genes in an unique chromatin conformation, and inhibit the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 expressed abundantly

Publikationen

Species	Application	Title
human	WB	J Exp Med; High-mobility group nucleosome-binding protein 1 acts as an alarmin and is critical for lipopolysaccharide-induced immune responses.; Authors - Yang De D
mouse	WB	Nat Commun; Alarmin-painted exosomes elicit persistent antitumor immunity in large established tumors in mice.; Authors - Bingfeng Zuo
human	WB	Cancer Res; The alarmin HMGN1 contributes to anti-tumor immunity and is a potent immunoadjuvant.; Authors - Feng Wei
mouse	WB	Theranostics; Alarmin augments the antitumor immunity of lentiviral vaccine in ectopic, orthotopic and autochthonous hepatocellular carcinoma mice.; Authors - Zhili Liu
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
mouse	IHC	PeerJ; PM2.5 exposure aggravates kidney damage by facilitating the lipid metabolism disorder in diabetic mice; Authors - Yuecheng Jiang