HMGCR Polyklonaler Antikörper

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• KD/KO Validated

HMGCR Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

45

Rezensionen (1)

Kat-Nr. : 13533-1-AP

Synonyme

HMG CoA reductase, HMGCR

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Galerie der Validierungsdaten

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  IHC staining of human liver cancer using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13533-1-AP (HMGCR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13533-1-AP (HMGCR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13533-1-AP (HMGCR antibody) at dilution of 1:1000 (under 10x lens)..

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  IHC staining of human small intestine using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13533-1-AP (HMGCR antibody) at dilution of 1:1000 (under 40x lens)..

• 

  IHC staining of human heart using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13533-1-AP (HMGCR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13533-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13533-1-AP (HMGCR Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Dünndarmgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 9 publications below
• IF: See 3 publications below

Produktinformation

13533-1-AP bindet in IF, IHC, ELISA HMGCR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGCR fusion protein Ag4444
• Vollständiger Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• Berechnetes Molekulargewicht: 888 aa, 95 kDa
• GenBank-Zugangsnummer: BC033692
• Gene symbol: HMGCR
• Gene ID (NCBI): 3156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMGCR(3-hydroxy-3-methylglutaryl-coenzyme A reductase) is also named as HMG-CoA reductase and belongs to the HMG-CoA reductase family. This protein exists as a 97-kDa glycoprotein in the endoplasmic reticulum (ER) and is the rate-determining enzyme of the biosynthesis pathway(PMID:15830340).The ectopic expression of either full-length HMGCR or its novel splice variant promotes dysregulation of the MVA pathway. And the ectopic expression of cHMGCR-FL(60 kDa) and cHMGCR-D13(55 kDa) can be detected in HepG2 cells(PMID:20696928).This protein is also can exsit as a oligomer(PMID:20696928).

Publikationen

Species	Application	Title
mouse	IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	IF	J Cell Biol; TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.; Authors - Wan Yun Ho ; KD Validated
human	IHC	Acta Pharmacol Sin; Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.; Authors - Hai-Yan Wang ; KD Validated
human		Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
human		PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
human	IHC	Mol Metab; LDLR inhibition promotes hepatocellular carcinoma proliferation and metastasis by elevating intracellular cholesterol synthesis through the MEK/ERK signaling pathway.; Authors - Ziye Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Malak (Verified Customer) (11-30-2020)

The antibody works very well in IHC staining especially at pH6 and with 1/100 dilution

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Hepatic tissue