HMG20A Polyklonaler Antikörper

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• KD/KO Validated

HMG20A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 12085-2-AP

Synonyme

HMGX1, HMG domain-containing protein HMGX1, HMG domain-containing protein 1, HMG box-containing protein 20A, HMG box containing protein 20A

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12085-2-AP

  WB result of HMG20A antibody (12085-2-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20A transfected HEK-293 cells.

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  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12085-2-AP

  IP result of anti-HMG20A (IP:12085-2-AP, 4ug; Detection:12085-2-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 12085-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12085-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12085-2-AP (HMG20A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12085-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA HMG20A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMG20A fusion protein Ag2718
• Vollständiger Name: high-mobility group 20A
• Berechnetes Molekulargewicht: 347 aa, 40 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC021959
• Gene symbol: HMG20A
• Gene ID (NCBI): 10363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation

Publikationen

Species	Application	Title
human	WB,IP	Nat Commun; The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs; Authors - Andreas Herchenröther ; KD Validated
human	WB	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	WB	EMBO Mol Med; LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.; Authors - Lukas Leiendecker
mouse	WB	Cancer Cell Int; Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p.; Authors - Yu Fu
human	IHC	Biochim Biophys Acta; Interactome of the inhibitory isoform of the nuclear transporter Importin 13.; Authors - Shadma Fatima
mouse	WB,IHC,IF,IP	Biochim Biophys Acta; The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins.; Authors - Davies Rebecca G RG