HLA-E Monoklonaler Antikörper

HLA-E Monoklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1A4G3

Kat-Nr. : 66530-1-Ig

Synonyme

EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

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Galerie der Validierungsdaten

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  WB analysis of THP-1 using 66530-1-Ig

  THP-1, Ramos, Daudi, and Raji cells were subjected to SDS PAGE followed by western blot with 66530-1-Ig (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66530-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66530-1-Ig (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66530-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66530-1-Ig (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66530-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66530-1-Ig (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66530-1-Ig (HLA-E antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66530-1-Ig (HLA-E antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66530-1-Ig (HLA-E antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66530-1-Ig (HLA-E antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HLA-E antibody (66530-1-Ig, Clone: 1A4G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of THP-1 using 66530-1-Ig

  1X10^6 THP-1 cells were stained with 0.20ug HLA-E antibody (66530-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, Daudi-Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, Jurkat-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2500-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

66530-1-Ig bindet in WB, IHC, IF, FC, ELISA HLA-E und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HLA-E fusion protein Ag6724
• Vollständiger Name: major histocompatibility complex, class I, E
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC002578
• Gene symbol: HLA-E
• Gene ID (NCBI): 3133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Immune checkpoint HLA-E:CD94-NKG2A mediates evasion of circulating tumor cells from NK cell surveillance; Authors - Xiaowei Liu ; KD Validated