HLA class I ABC Polyklonaler Antikörper

HLA class I ABC Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Rezensionen (2)

Kat-Nr. : 15240-1-AP

Synonyme

HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA-A, MHC class I antigen A*3

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Galerie der Validierungsdaten

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  WB analysis using 15240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15240-1-AP (HLA class I ABC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15240-1-AP

  IP Result of anti-HLA class I ABC (IP:15240-1-AP, 3ug; Detection:15240-1-AP 1:500) with HepG2 cells lysate 1720ug.

• 

  IHC staining of human tonsillitis using 15240-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15240-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15240-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15240-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15240-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15240-1-AP

  1X10^6 HepG2 cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (15240-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 11 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

15240-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA HLA class I ABC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLA class I ABC fusion protein Ag7370
• Vollständiger Name: major histocompatibility complex, class I, A
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC003069
• Gene symbol: HLA-A
• Gene ID (NCBI): 3105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Publikationen

Species	Application	Title
human	WB	Nature; Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.; Authors - Xinjian Liu
human	WB	Cell; Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.; Authors - Kazuhiro Koikawa
human		Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
	WB	ACS Appl Mater Interfaces; Self-Assembly of a Multifunction DNA Tetrahedron for Effective Delivery of Aptamer PL1 and Pcsk9 siRNA Potentiate Immune Checkpoint Therapy for Colorectal Cancer.; Authors - Wenjing Guo
human	WB	J Immunother Cancer; MYC suppresses STING-dependent innate immunity by transcriptionally upregulating DNMT1 in triple-negative breast cancer.; Authors - Si-Yu Wu
human	WB	J Immunother Cancer; THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.; Authors - Chushu Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fabien (Verified Customer) (11-04-2022)

The antibody works great for western-blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Jy cells

FH

Patricia (Verified Customer) (02-25-2020)

I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000 for WB; 4-10 ug/mL IP
• Cell Tissue Type: Ovarian cancer

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